Technical Data
041805
SLC16A3, CT (SLC16A3, MCT4, Monocarboxylate transporter 4, Solute carrier family 16 member 3)
Description:
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100-1:500
Immunohistochemistry (FFPE): 1:10-1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
NCI-H460 cell lysates, human skeletal muscle

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a portion of amino acids within aa440-465 from the C-terminla region of human SLC16A3, conjugated to KLH (O35910) (Gene ID 9123) Cellular Localization: Cell Membrane; Multi-pass membrane protein
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human SLC16A at ~50kD. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Vellonen, K.S., et al. Eur J Pharm Sci 39(4):241-247(2010). 3. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 4. Wang, Q., et al. Drug Metab. Dispos. 35(8):1393-1399(2007). 5. Olsen, J.V., et al. Cell 127(3):635-648(2006)