Technical Data
042020-APC
SMAD2, phosphorylated (T220) (SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2) (APC)
Description:
SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.

Applications:
Suitable for use in Immunofluorescence, Dot Blot, FLISA

Recommended Dilution:
FLISA: 1:1,000
Dot blot 1:500
Immunofluorescence: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Phospho-SMAD2-T220 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 208~238 amino acids surrounding T220 of human SMAD2.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Papageorgis,P., et.al., Cancer Res. 70 (3), 968-978 (2010)
Funaba,M., et.al., J. Biol. Chem. 277 (44), 41361-41368 (2002)