Technical Data
SMAD3 (S208) (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3)
SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Immunofluorescence: 1:10-1:50
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As Reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 186-215 of human SMAD3, UniProt Accession #P84022. Species Sequence Homology: chicken, porcine and rat
Purified by Protein A affinity chromatography
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Recognizes human SMAD3. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang, M., et al. J. Biol. Chem. 285(12):8703-8710(2010). 2. Daly, A.C., et al. J. Biol. Chem. 285(9):6489-6497(2010). 3. Heikkinen, P.T., et al. J. Biol. Chem. 285(6):3740-3749(2010). 4. Tseng, Z.H., et al. Heart Rhythm 6(12):1745-1750(2009)