Technical Data
042023
SMAD3, ID (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3)
Description:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20°CBlue IceHumanRabbit
Concentration:
0.25mg/ml
Immunogen:
SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-156 amino acids from the Central region of human SMAD3.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Ge, Q., et al. J. Cell. Physiol. 225(3):846-854(2010)
Lee, J., et al. J. Biol. Chem. 285(34):26618-26627(2010)
Roder, C., et al. Childs Nerv Syst (2010) In press :
Valdes, A.M., et al. Arthritis Rheum. 62(8):2347-2352(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :