Technical Data
043128-PE
TOP2A, CT (TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme) (PE)
Description:
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, FLISA

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1174~1204 amino acids from the C-terminal region of human TOP2A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kawamura,R., et.al, J. Cell Biol. 188 (5), 653-663 (2010)
Alchanati,I., et.al, PLoS ONE 4 (12), E8104 (2009)