Technical Data
043219
TRIM23, NT (TRIM23, ARD1, ARFD1, RNF46, E3 ubiquitin-protein ligase TRIM23, ADP-ribosylation factor domain-containing protein 1, GTP-binding protein ARD-1, RING finger protein 46, Tripartite motif-containing protein 23)
Description:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
TRIM23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the N-terminal region of human TRIM23.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Arimoto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 107(36):15856-15861(2010)
Poole, E., et al. J. Virol. 83(8):3581-3590(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Vichi, A., et al. Proc. Natl. Acad. Sci. U.S.A. 102(6):1945-1950(2005)
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)