Technical Data
043223
TRIM27, CT (TRIM27, RFP, RNF76, Zinc finger protein RFP, RING finger protein 76, Ret finger protein, Tripartite motif-containing protein 27)
Description:
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
TRIM27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-482 amino acids from the C-terminal region of human TRIM27.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Johnson, A.D., et al. Nat. Genet. 42(7):608-613(2010)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Tezel, G.G., et al. Pathol. Res. Pract. 205(6):403-408(2009)
Li, X., et al. Virology 360(2):419-433(2007)
Lim, J., et al. Cell 125(4):801-814(2006)