Technical Data
TTL, CT (TTL, Tubulin-tyrosine ligase)
TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).

Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-385 amino acids from the C-terminal region of human TTL.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :
Erck, C., et al. Cytogenet. Genome Res. 101(1):47-53(2003)
Mialhe, A., et al. Cancer Res. 61(13):5024-5027(2001)
Ersfeld, K., et al. J. Cell Biol. 120(3):725-732(1993)