Technical Data
044562
APRIL, blocking (Recombinant) (A Proliferation Inducing Ligand, TNFSF13, CD256, TALL-2)
Description:
APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. Anti-APRIL (mouse) Monoclonal Antibody (recombinant) (Blocking) (APRY-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Applications:
Suitable for use in ELISA, Neutralization, Immunoprecipitation

Recommended Dilution:
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,lApry-1-1Highly Purified
SizeStorageShippingSourceHost
100ug4C/-20CBlue IceMouseMouse
Concentration:
1mg/ml
Immunogen:
Mouse APRIL (aa 98-232).
Purity:
>95% (SDS-PAGE)
Form
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Specificity:
Recognizes mouse APRIL. Does not recognize mouse BAFF.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Product Reference:
Production of the plasma-cell survival factor APRIL peaks in myeloid precursor cells from human bone marrow: T. Matthes, et al.; Blood (Epub ahead of print) (2011)