Technical Data
APRIL, blocking (Recombinant) (A Proliferation Inducing Ligand, TNFSF13, CD256, TALL-2)
APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. Anti-APRIL (mouse) Monoclonal Antibody (recombinant) (Blocking) (APRY-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Suitable for use in ELISA, Neutralization and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation: 1:200
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2bApry-1-1Highly Purified
100ug-20CBlue IceMouseMouse
Recombiant protein corresponding to aa98-232 from mouse APRIL
>95% (SDS-PAGE) Endotoxin: <0.1EU/ug purified protein (LAL)
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Recognizes mouse APRIL. Does not recognize mouse BAFF. Inhibits the binding of mouse APRIL to BCMA and TACI.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Production of the plasma-cell survival factor APRIL peaks in myeloid precursor cells from human bone marrow: T. Matthes, et al.; Blood (Epub ahead of print) (2011)