Bisindolylmaleimide IX methanesulfonate (Ro 31-8220, BIM IX)
|Biochemicals||Storage: 4°C/-20°CShipping: Blue Ice|
Selective and cell permeable protein kinase C (PKC) inhibitor. Inhibits the stimulation of insulin secretion by glucose. Inhibits T cell activation. Apoptosis inducer. Potent glycogen synthase kinase-3 (GSK-3) inhibitor. Transcription inhibitor. Induces TNF receptor family-mediated cell death. Pim-1 kinase inhibitor. Antitumor compound.
Storage and Stability:
Short-term Storage: +4°C
Long-term Storage: -20°C
Stable for at least 2 years after receipt when stored at -20°C. Store solutions at -20°C in the dark.
C25H23N5O2S . CH4O3S
Purity: >98% (NMR)
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Potent selective inhibitors of protein kinase C: P.D. Davis, et al.; FEBS Lett. 259, 61 (1989)
RO 31-8220 and RO 31-7549 show improved selectivity for protein kinase C over staurosporine in macrophages: P. Dieter & E. Fitzke; BBRC 181, 396 (1991)
Isoenzyme specificity of bisindolylmaleimides, selective inhibitors of protein kinase C: S.E. Wilkinson, et al.; Biochem. J. 294, 335 (1993)
Inhibition of glucose-stimulated insulin secretion by Ro 31-8220, a protein kinase C inhibitor: S.J. Persaud & P.M. Jones; Endocrine 3, 285 (1995)
RO 31-8220, a novel protein kinase C inhibitor, inhibits early and late T cell activation events: L. Geiselhart, et al.; Transplantation 61, 1637 (1996)
Induction of apoptosis in glioblastoma cells by inhibition of protein kinase C and its association with the rapid accumulation of p53 and induction of the insulin-like growth factor-1-binding protein-3: L. Shen & R.I. Glazer; Biochem. Pharmacol. 55, 1711 (1998)
The protein kinase C inhibitors bisindolylmaleimide I (GF 109203x) and IX (Ro 31-8220) are potent inhibitors of glycogen synthase kinase-3 activity: I. Hers, et al.; FEBS Lett. 460, 433 (1999)
The staurosporine analog, Ro-31-8220, induces apoptosis independently of its ability to inhibit protein kinase C: Z. Han, et al.; Cell Death Differ. 7, 521 (2000)
Bisindolylmaleimide IX facilitates tumor necrosis factor receptor family-mediated cell death and acts as an inhibitor of transcription: O.W. Rokhlin, et al.; J. Biol. Chem. 277, 33213 (2002)
Bisindolylmaleimide IX Induces Reversible and Time-Dependent Tumor Necrosis Factor Receptor Family-Mediated Caspase Activation and Cell Death: O.W. Rokhlin & M.B. Cohen; Cancer Biol. Ther. 2, 266 (2003)
Protein kinase C-independent effects of protein kinase D3 in glucose transport in L6 myotubes: J. Chen, et al.; Mol. Pharmacol. 67, 152 (2005)
Pim-1 ligand-bound structures reveal the mechanism of serine/threonine kinase inhibition by LY294002: M.D. Jacobs, et al.; J. Biol. Chem. 280, 13728 (2005)
Bisindolylmaleimide IX facilitates extrinsic and initiates intrinsic apoptosis in TNF-alpha-resistant human colon adenocarcinoma COLO 205 cells: B. Pajak, et al.; Apoptosis 13, 509 (2008)