Technical Data
Bisindolylmaleimide VIII acetate (Ro 31-7549, BIM VIII)
Biochemicals Storage: 4C/-20CShipping: Blue Ice
Selective protein kinase C (PKC) inhibitor. Inhibits proliferation of cultured mouse epidermal keratinocytes. Enhances Fas- and TRAIL-mediated apoptosis. Inhibits T cell-mediated autoimmune diseases. Inhibits stimulation of insulin secretion by glucose. Inhibits panel of protein kinases (e.g. GSK-3beta, CDK2).

Storage and Stability:
Short-term Storage: +4C
Long-term Storage: -20C
Stable for at least 2 years after receipt when stored at -20C. Store solutions at -20C in the dark.

CAS Number:

Molecular Formula:
C24H22N4O2 . CH3COOH

Molecular Weight:
Purity: >98% (NMR)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Product Reference:
RO 31-8220 and RO 31-7549 show improved selectivity for protein kinase C over staurosporine in macrophages: P. Dieter & E. Fitzke; BBRC 181, 396 (1991)
Isoenzyme specificity of bisindolylmaleimides, selective inhibitors of protein kinase C: S.E. Wilkinson, et al.; Biochem. J. 294, 335 (1993)
Inhibitors of protein kinase C. 3. Potent and highly selective bisindolylmaleimides by conformational restriction: R.A. Bit, et al.; J. Med. Chem. 36, 21 (1993)
Effects of the selective protein kinase C inhibitor, Ro 31-7549, on the proliferation of cultured mouse epidermal keratinocytes: W.B. Bollag, et al.; J. Invest. Dermatol. 100, 240 (1993)
Bisindolylmaleimide VIII facilitates Fas-mediated apoptosis and inhibits T cell-mediated autoimmune diseases: T. Zhou, et al.; Nat. Med. 5, 42 (1999)
Bisindolylmaleimide VIII enhances DR5-mediated apoptosis through the MKK4/JNK/p38 kinase and the mitochondrial pathways: T. Ohtsuka & T. Zhou; J. Biol. Chem. 277, 29294 (2002)
Proteome-wide identification of cellular targets affected by bisindolylmaleimide-type protein kinase C inhibitors: D. Brehmer, et al.; Mol. Cell Proteomics 3, 490 (2004)
Different signaling pathways involved in glucose- and cell swelling-induced insulin secretion by rat pancreatic islets in vitro: Z. Bacova, et al.; Cell Physiol. Biochem. 16, 59 (2005)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.