Technical Data
H-89 dihydrochloride (N-5-isoquinolinesulfonamide . 2HCl)
Biochemicals Storage: +4C/+4CShipping: Blue Ice
Cell permeable potent and selective cAMP- and cGMP-dependent protein kinase (PKA and PKG) inhibitor. Protein kinase C (PKC) inhibitor. Ca2+/calmodulin-dependent protein kinase II inhibitor. Casein kinase I inhibitor. Myosin light chain kinase (MLCK) inhibitor. Apoptosis enhancer. Tool to study protein crystal structure-inhibitor interactions. Rho kinase inhibitor. Cell proliferation inhibitor. Review.

Storage and Stability:
Short-term Storage: +4C
Long-term Storage: +4C
Stable for at least 2 years after receipt when stored at +4C.

CAS Number:

Molecular Formula:
C20H20BrN3O2S . 2HCl

Molecular Weight:
Purity: >98% (NMR)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Product Reference:
Polyamines differentially inhibit cyclic AMP-dependent protein kinase-mediated phosphorylation in the brain of the tobacco hornworm, Manduca sexta.: W.L. Combest et al.; J. Neurochem. 51, 1581 (1988)
Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N--5-isoquinolinesulfonamide (H-89), of PC12: T. Chijiwa et al.; J. Biol. Chem. 265, 5267 (1990)
A selective inhibitor of cyclic AMP-dependent protein kinase, N--5- isoquinolinesulfonamide (H-89), inhibits phosphatidylcholine biosynthesis in HeLa cells: C.C. Geilen et al.; FEBS Lett. 309, 381 (1992)
Characterization of activators and inhibitors of protein kinase C mu: F.J. Johannes et al.; Eur. J. Biochem. 227, 303 (1995)
Protein kinase A inhibitors enhance radiation-induced apoptosis: D. Findik et al.; J. Cell Biochem. 57, 12 (1995)
Crystal structures of catalytic subunit of cAMP-dependent protein kinase in complex with isoquinolinesulfonyl protein kinase inhibitors H7, H8, and H89. Structural implications for selectivity: R.A. Engh et al.; J. Biol. Chem. 271, 26157 (1996)
The protein kinase A inhibitor H89 acts on cell morphology by inhibiting Rho kinase: J. Leemhuis, et al.; J. Pharmacol. Exp. Ther. 300, 1000 (2002)
H89 (N--5-isoquinolinesulfonamide) induces reduction of myosin regulatory light chain phosphorylation and inhibits cell proliferation: D. Umeda, et al.; Eur. J. Pharmacol. 590, 61 (2008)
The many faces of H89: a review: A. Lochner & J.A. Moolman; Cardiovasc. Drug Rev. 24, 261 (2006)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.