Technical Data
IL-33 (Interleukin-33, IL-1F11, NF-HEV)
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kD human IL33 is converted by CASP1 to a 18kD protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Direct and Indirect): 1:2000-1:5000
Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween-20 and 5% skim milk; suggested incubation time is 1 hour at RT.
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Recombinant mouse IL-33.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Recognizes mouse IL-33. Detects a band of ~18kD by Western Blot. Does not cross-react with human IL-33.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.