Technical Data
LIF, Recombinant, Human (Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor)
Molecular Biology Storage: 4C/-20CShipping: Blue Ice
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kD (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.

Biological Activity:
Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.

Human LIF (aa 23-202) is fused at the N-terminus to a GST-tag.

Storage and Stability:
Short-term Storage: +4C
Long-term Storage: -20C
Stable for at least 6 months after receipt when stored at -20C.

Molecular Weight:
~46kD (SDS-PAGE)
Source: Human, E. coli
Purity: >95% (SDS-PAGE)
Concentration: 0.1mg/ml/1mg/ml
Form: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS pH 7.2.
Specificity: Mouse and human embryonic stem cells.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.