Technical Data
045087
NLRP3/NALP3 (NACHT-, LRR- and PYD-containing Protein 3, NLRP3, Cryopyrin, Cold Autoinflammatory Syndrome 1 Protein)
Description:
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin-1beta (IL-1b) and IL-18. Members of the Nod-like receptor family, including NLRP3 / NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2bCryo-1Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Specificity:
Recognizes mouse NLRP3/NALP3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.