Technical Data
Polyglutamylation Modification (Biotin) (Polyglutamylated Tubulin, Glutamylated Tubulin, Postranslational Protein Glutamylation)
Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved from and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and involved in cell cycle and cell proliferation.

Suitable for use in Immunocytochemistry, Immunoprecipitation, Western Blot

Recommended Dilution:
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,kGT335Highly Purified
100ug4C/-20CBlue IceMouse
Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
>95% (SDS-PAGE)
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Product Reference:
Distribution of glutamylated alpha and beta-tubulin in mouse tissues using a specific monoclonal antibody, GT335: A. Wolff, et al.; Eur. J. Cell Biol. 59, 425 (1992)
Polyglutamylation of nucleosome assembly proteins: C. Regnard, et al.; J. Biol. Chem. 275, 15969 (2000)
Polyglutamylation Is a Post-translational Modification with a Broad Range of Substrates: J. van Dijk, et al.; J. Biol. Chem. 283, 3915 (2008)