|Biochemicals||Storage: 4°C/-20°CShipping: Blue Ice|
Cell permeable smoothened (Smo) agonist. Shown to induce Sonic hedgehog (Shh) pathway activation and counteracts cyclopamine inhibition of Smo. Acts as an activator of Smo at low concentrations and as an inhibitor of Smo at very high concentrations.
Storage and Stability:
Short-term Storage: +4°C
Long-term Storage: -20°C
Stable for at least 2 years after receipt when stored at -20°C. Store solutions at -20°C in the dark.
Purity: >98% (NMR)
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists: M. Frank-Kamenetsky, et al.; J. Biol. 1, 10 (2002)
Small molecule modulation of Smoothened activity: J.K. Chen, et al.; PNAS 99, 14071 (2002)
Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2: W. Chen, et al.; Science 306, 2257 (2004)
Smoothened Signal Transduction is Promoted by G-Protein Coupled Receptor Kinase 2: A.R. Meloni et al.; Mol. Cell Biol. 26, 7550 (2006)
Oxysterols stimulate Sonic hedgehog signal transduction and proliferation of medulloblastoma cells: R.B. Corcoran & M.P. Scott; PNAS 103, 8408 (2006)
Identification and characterization of Hedgehog modulator properties after functional coupling of Smoothened to G15: C. Masdeu, et al.; BBRC 349, 471 (2006)
Wnt signaling stimulates transcriptional outcome of the Hedgehog pathway by stabilizing GLI1 mRNA: F.K. Noubissi, et al.; Cancer Res. 69, 8572 (2009)
Evidence for allosteric interactions of antagonist binding to the smoothened receptor: C.M. Rominger, et al.; J. Pharmacol. Exp. Ther. 329, 995 (2009)
Converse conformational control of smoothened activity by structurally related small molecules: H. Yang, et al.; J. Biol. Chem. 284, 20876 (2009)
Identification of a suppressive mechanism for Hedgehog signaling through a novel interaction of Gli with 14-3-3: Y. Asoaka, et al.; J. Biol. Chem. 285, 4185 (2010)
Smoothened agonist augments proliferation and survival of neural cells: O. Bragina, et al.; Neurosci. Lett. 482, 81 (2010)
Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals: T. Danjo, et al.; J. Neurosci. 31, 1919 (2011)