Technical Data
045397
Z-VAD-FMK (Cell permeable) (Z-VAD(OMe)-FMK, pan-Caspase Inhibitor, Z-Val-Ala-DL-Asp(OMe)-fluoromethylketone, Benzyloxycarbonyl-Val-Ala-DL-Asp(OMe)-fluoromethylketone)
1mg
Biochemicals Storage: 4C/-20CShipping: Blue Ice
Cell-permeable, non-selective broad-spectrum caspase inhibitor. Binds irreversibly to the catalytic site of caspase proteases.
The peptide is O-methylated in the P1 position on aspartic acid, providing enhanced stability and increased cell permeability.
Inhibits ICE-family protease/caspase processing, leading to apoptosis and autophagy induction.
Decreases proteasome activity.
Potent inhibitor of caspase-1 activation in NLRP3-induced cells.
Used in apoptosis and inflammasome studies.


Storage and Stability:
Short-term Storage: +4C
Long-term Storage: -20C
Stable for at least 3 years after receipt when stored at -20C.

CAS Number:
187389-52-2,634911-81-2

Molecular Formula:
C22H30FN3O7

Molecular Weight:
467.5
Purity: >95% (HPLC)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Product Reference:
Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32: E.A. Slee, et al.; Biochem. J. 315, 21 (1996)
Different interleukin-1 beta converting enzyme (ICE) family protease requirements for the apoptotic death of T lymphocytes triggered by diverse stimuli: A. Sarin, et al.; J. Exp. Med. 184, 2445 (1996)
Processing/activation of at least four interleukin-1beta converting enzyme-like proteases occurs during the execution phase of apoptosis in human monocytic tumor cells: M. MacFarlane, et al.; J. Cell Biol. 137, 469 (1997)
Processing/activation of caspases, -3 and -7 and -8 but not caspase-2, in the induction of apoptosis in B-chronic lymphocytic leukemia cells: D. King, et al.; Leukemia 12, 1553 (1998)
Proteasome activities decrease during dexamethasone-induced apoptosis of thymocytes: J. Beyette, et al.; Biochem. J. 332, 315 (1998)
JNK (c-Jun N-terminal kinase) and p38 activation in receptor-mediated and chemically-induced apoptosis of T-cells: differential requirements for caspase activation: M. MacFarlane, et al.; Biochem. J. 348, 93 (2000)
Statin-induced proinflammatory response in mitogen-activated peripheral blood mononuclear cells through the activation of caspase-1 and IL-18 secretion in monocytes: W.R. Coward, et al.; J. Immunol. 176, 5284 (2006)
Broad-spectrum caspase inhibitors: from myth to reality? D. Chauvier, et al.; Cell Death Differ. 14, 387 (2007)
A calpain-like protease inhibits autophagic cell death: D.T. Madden, et al.; Autophagy 3, 519- (2007)
Malarial hemozoin is a Nalp3 inflammasome activating danger signal; C. Dostert, et al.; PLoS One 4, e6510 (2009)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.