Technical Data
045406
APOA1 (Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Apolipoprotein A-I(1-242))
Description:
Apolipoprotein A1 (APOA1) is the main protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in APOA1 are associated with HDL deficiencies. High level of APOA1 is regarded as protective against atherosclerosis and cardiovascular disease.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Hybridoma:
F0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a, k12L8Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to human APOA1 (25-267aa) expressed in E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human APOA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dastani Z, et al. (March 2006). Atherosclerosis 185 (1): 127-36. 2. Chiesa G, Sirtori CR (2003). Curr. Opin. Lipidol. 14 (2): 159-63. 3. Pussinen, et al. (Jan. 1998). J. Lipid Res. (UNITED STATES) 39 (1): 152-61.