Technical Data
045423
EGF, Recombinant, Mouse, aa977-1029, His-Tag (Pro-epidermal Growth Factor, Epidermal Growth Factor)
20ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Recombinant mouse epidermal growth factor (EGF) is a 8.6kD globular protein containing 77aa residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.

Source:
Recombinant corresponding to aa977-1029 from mouse EGF, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~8.6kD (77aa) confirmed by MALDI-TOF

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~8.6kD
Source: E.coli
Purity: ~90% (SDS-PAGE)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 100mM sodium chloride, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Riese., et al (1998 ) Bioessays. 20: 41-48. 2. Cohen S., (1983) Cancer. 15: 1787-1791. 3. Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.