Technical Data
045493
SF2 (SRSF1, ASF, FLJ53078, MGC5228, SF2, SF2p33, SFRS1, SRp30a, Serine/arginine-rich splicing factor 1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit)
Description:
SF2 is a member of the arginine/serine-rich splicing factor protein family and functions in both constitutive and alternative pre-mRNA splicing. It binds to pre-mRNA transcripts and components of the spliceosome, and it can either activate or repress splicing depending on the location of the pre-mRNA binding site. SF2's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins (1).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-10,000
Immunohistochemistry: 1:50-100
Immunocytochemistry: 1:50-100
Immunoprecipitation: 1:10-100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
Mab13C110Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in human SF2.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, sodium azide, BSA, glycerol.
Specificity:
Recognizes human SF2. Species sequence homology: Mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.