Technical Data
045632
IDE (Insulin-degrading Enzyme, Abeta-degrading Protease, Insulin Protease, Insulinase, Insulysin)
Description:
IDE is a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulin's activity, as well as participating in intercellular peptide signaling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in IDE's function are associated with Alzheimer's disease and type 2 diabetes mellitus, but mutations in IDE have not been shown to be causative for these diseases. It localizes primarily in the cytoplasm, but in some cell types it localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabEPR6098(2)Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in human IDE.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Specificity:
Recognizes human IDE. Species crossreactivity: Mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.