Technical Data
131110
PDLIM7 (PDZ and LIM Domain Protein 7, LIM Mineralization Protein, LMP, Protein Enigma, ENIGMA)
Description:
PDLIM7 (PDZ and LIM domain 7) is a representative of a family of proteins composed of conserved PDZ and LIM domains. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. It is likely to act as a scaffold for the formation of multiprotein complexes. It may play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. PDLIM7 is involved in the fundamental mechanisms of bone formation and is involved in the BMP signaling pathway. There are six isoforms.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 10ug/ml
Optimal dilutions to be determined by the researcher.

AA Sequence:
MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGVPYCERDYEKMFGTKCHGCDFKIDAGDRFLEALGFSWHDTCFVCAICQINLEGKTFYSKKDRPLCKSHAFSHV

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2D6Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Full length recombinant corresponding to aa1-457 from PDLIM7 (AAH01093) with GST tag. MW of the GST tag alone is 26kD.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human PDLIM7.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Postsynaptic PDLIM5/Enigma Homolog binds SPAR and causes dendritic spine shrinkage. Herrick S, Evers DM, Lee JY, Udagawa N, Pak DT.Mol Cell Neurosci. 2009 Nov 10. [Epub ahead of print]