Technical Data
136283
NEFL (Neurofilament Light Polypeptide, NF-L, NF68, CMT2E, FLJ53642)
Description:
Neurofilaments are the intermediate filaments found in neuronal cells. They are composed of a combination of subunit proteins which typically includes the neurofilment triplet proteins, NF-L (68kD), NF-M (160kD), and NF-H (200kD). Neurofilaments may also include peripherin, alpha-internexin (NF66), nestin, and vimentin. Antibodies to the various neurofilament subunits are useful biomarkers; the filaments are abundant and biochemically stable. The expression of neurofilament proteins varies during neuronal development and differentiation and between between neuronal cell subtypes, and altered in pathological states. Accumulation of neurofilaments is observed the progression of numerous neurological diseases.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:1000
Immunohistochemistry (Paraffin): 10ug/ml
Immunohistochemistry (Frozen): 1:25-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap..
TypeIsotypeCloneGrade
MabIgG1Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to human NEFL/NF-L.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human NEFL/NF-L.. Species Crossreactivity: Opossum
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.