Technical Data
136860
Adenylate Cyclase Type 3 (Adenylate Cyclase Type III, AC-III, Adenylyl Cyclase 3, AC3, Adcy3, Adenylate Cyclase Olfactive Type, ATP Pyrophosphate-lyase 3)
Description:
Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins. Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP. The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons.

Adenylate cyclase type III is a large complex molecule of, in the human, 1145aa with a deduced molecular weight of 129kD. The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160kD in cortex and about 200kD in olfactory epithelium. The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains. Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:500-1:1000
Western Blot: 1:10,000 (major bands at 160-200kD, depending on the tissue)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to fusion protein containing the extreme C-terminal segment of rat NF-M.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid.
Specificity:
Recognizes rat ADCY3. Species Crossreactivity: feline, chicken, human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.