Technical Data
138563
Lambda Chain (B cells)
Description:
This monoclonal antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1-2ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.

Positive Control:
293T, Raji or hPBL cells. Tonsil or Spleen

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kICO-106Affinity Purified
SizeStorageShippingSourceHost
20ug-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified human IgG
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138563AF.
Specificity:
Recognizes human lambda.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Leung N et. al. Leuk Lymphoma doi:10. 3 109/10428194.2012.67 3 229 (2012). 2. Villaverde et al. Clin Kidney J 5:59-62 (2012).