Technical Data
140038
BD-2 (Beta-defensin 4A, Beta-defensin 2, hBD-2, Defensin, beta 2, Skin-antimicrobial Peptide 1, SAP1, DEFB4A, DEFB102, DEFB2, DEFB4, DEFB4B)
Description:
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:100-1:200
Western Blot: 1:50-1:400
Immunohistochemistry (Formalin fixed frozen sections): 1:50-1:500
Immunohistochemistry (Paraffin): 1:10-1:100
Immunocytochemistry (Formalin fixed cells): 1:50-1:500
Optimal dilutions to be determined by the researcher.

Amino Acid Sequence:
MGHHHHHHSGSEF-GIGDPVT CLKSGAICHP VFCPRRYKQI GTCGLPGTKC CKKP

Included Protein Molecular Weight Marker:
168631: Unstained Protein Molecular Weight Marker, 10-70kD
Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.

Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD.
Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.

Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:
Mini gels: 3-5ul/well
Large gel: 7ul/well
The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Recombinant protein corresponding to aa24-64 with two N-Terminal Tags, His-tag and GST-Tag from human DEFb2, expressed in E. coli
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes human Defensin Beta 2 (DEFb2)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.