Technical Data
142854
CD40 Ligand, Recombinant, Human, Animal Free (CD-40-Ligand, AF, TNFSF5, TRAP, CD154, gp39, T-BAM)
10ug
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-y, TNF-a, and IL-2.

Source:
Recombinant protein corresponding to 149aa from human CD40 Ligand expressed in E.coli.

Molecular Weight:
~16.3kD

Endotoxin:
1EU/ug (LAL)

Biological Activity:
The activity is determined by the dose production of IL-8 by human PBMCs and is typically 5-10ng/ml.

AA Sequence:
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~16.3kD
Source: E.coli
Purity: ~98% (HPLC, Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder in 10mM Na2PO4, pH 7.5. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.