Technical Data
142875
Follistatin, Recombinant, Mouse (FS, Activin-binding Protein, FSH-suppressing Protein, FSP)
5ug
20ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors.

Source:
Recombinant protein corresponding to 289aa from mouse follistatin expressed in E.coli.

Molecular Weight:
~31.6kD

Endotoxin:
1EU/ug (LAL)

Biological Activity:
The activity is determined by the dose-dependent neutralization of 7.5ng/ml human Activin A. Complete neutralization is typically reached at <0.3ug/ml.

AA Sequence:
MGNCWLRQAK NGRCQVLTKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DS

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~31.6kD
Source: E.coli
Purity: ~90% (Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder in 10mM Na2PO4, 50mM NaCl, pH 7.5. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.