Technical Data
142924
Interleukin 21, Recombinant, Human, Animal Free (IL-21AF)
2ug
10ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Interleukin-21 (IL-21) is a member of the common gamma-chain family of cytokines (includes IL-2, IL-4, IL-7, IL-9, IL-13, and IL- 15) and is expressed by activated CD4+ T cells. IL-21 has a private receptor known as IL-21R, which is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple Ab classes in vivo. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells.

Source:
Recombinant protein corresponding to 133aa from human Interleukin-21 expressed in E.coli.

Molecular Weight:
~15.5kD

Endotoxin Level:
1EU/ug (LAL)

Biological Activity:
The activity is determined by the dose-dependent stimulation of Mino cells and is typcially <0.1-0.6ng/ml.

AA Sequence:
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~15.5kD
Source: E.coli
Purity: ~95% (Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder in 20mM Na2PO4, pH 7.5. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.