Technical Data
142938
Interleukin 4, Recombinant, Human, Animal Free (IL-4, AF, B Cell Stimulating Factor, BSF-1, BCDF, BCGF)
5ug
20ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Interleukin-4 (IL-4) is an immuno-modulatory cytokine, produced primarily by a subgroup of helper T cells, known as Th2. IL-4 is known to induce nave helper T cells to differentiate to Th2 cells, which subsequently produce more IL-4. Th2 cells regulate a wide variety of other immune cells including, B cells, where it has the ability to promote IgG to IgE isotype switching.

Source:
Recombinant protein corresponding to 130aa from human IL-4 expressed in E.coli.

Molecular Weight:
~15kD

Endotoxin:
1EU/ug (LAL)

Biological Activity:
The activity is determined by a proliferation assay using MC-9 cells and is typically <0.2ng/ml.

AA Sequence:
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~15kD
Source: E.coli
Purity: ~98% (HPLC, Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.