Technical Data
142951
MCP-1, Recombinant, Mouse (Monocyte Chemotactic Protein-1, CCL2, JE, MCAF)
2ug
10ug
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Monocyte Chemotactic Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells.

Source:
Recombinant protein corresponding to 125aa from mouse MCP-1 expressed in E.coli.

Molecular Weight:
~13.8kD

Endotoxin:
1EU/ug (LAL)

Biological Activity:
The activity is determined by its ability to chemoattract human PBMCs or THP-1 cells at 4-15ng/ml.

AA Sequence:
QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN

Storage and Stability
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~13.8kD
Source: E.coli
Purity: ~98% (HPLC, Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.