Technical Data
142965
Platelet Derived Growth Factor AA, Recombinant, Animal Free, Human (AF, PDGF-AA, GDGF, ODGF)
2ug
10ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-a, TGF-b and EGF.

Source:
Recombinant protein corresponding to two 125aa chains from human PDGF- AA expressed in E.coli.

Molecular Weight:
~28.5kD

Endotoxin:
1EU/ug (LAL)

Biological Activity:
The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically 3-5ng/ml.

AA Sequence:
SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: ~97% (Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.