Technical Data
142966
Platelet Derived Growth Factor AA, Recombinant, Mouse (PDGF-AA, GDGF, ODGF)
2ug
10ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-a, TGF-b and EGF.

Source:
Recombinant protein corresponding to two 126aa chains mouse PDGF-AA expressed in E.coli.

Molecular Weight:
~28.9kD

Endotoxin:
1EU/ug (LAL)

Biological Activity:
The activity is determined by the dose-dependent proliferation of Balb/c 3T3 cells and is typically 8-10ng/ml.

AA Sequence:
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~28.9kD
Source: E.coli
Purity: ~97% (Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder. No additives. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.