Technical Data
142987
Thrombopoietin, Recombinant, Human, Animal Free (TPO, AF, Megakaryocyte Colony Stimulating Factor, c-MPL ligand, MGDF)
2ug
10ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Thrombopoietin (TPO) is a growth factor that is produced by liver and kidney tissue. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets.

Source:
Recombinant protein corresponding to 174aa from human TPO expressed in E.coli.

Molecular Weight:
~18.6kD

Endotoxin:
1EU/ug (LAL)

Biological Activity:
The activity is determined by the dose-dependent stimulation of MO7e cells and is typically <1ng/ml.

AA Sequence:
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18.6kD
Source: E.coli
Purity: ~95% (Reducing and non-reducing SDS-PAGE)
Form: Supplied as a lyophilized powder in 10mM Na2PO4, pH 8.0. Reconstitute with sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.