Technical Data
144728
IL-10 (Cytokine synthesis inhibitory factor)
Description:
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

UniProt Number:
P22301

Gene ID:
IL10

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry (Paraffin).

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Handling:
Store at -20 C for one year. After reconstitution, store at 4 C for one month. Can also be aliquoted and stored frozen at -20 C for long term.
Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C/-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
E. coli-derived human IL-10 recombinant protein (Position: S19-N178).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder. Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity:
Recognizes human IL-10. No crossreactivity with other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence".