Technical Data
145860
4-1BB Receptor Human BioAssay™ ELISA Kit
10 Plates
Kits and Assays Storage: 4°C/-20°CShipping: Blue Ice
Human 4-1BB Receptor ELISA Cytokine Development kit contains the key components required for the quantitative measurement of natural and/or recombinant Human 4-1BB Receptor in a sandwich ELISA format. Using the ELISA protocol described below, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay Human 4-1BB Receptor in approximately 1000 ELISA plate wells.

Detection Range:
16–2000pg/ml

Cross Reactivity:
As reported

Reconstitution and Storage:

Capture Antibody:
50ug of antigen-affinity purified rabbit anti-Human 4-1BB Receptor containing 2.5mg D-mannitol.
Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml.

Detection Antibody:
50ug of biotinylated antigen-affinity purified rabbit anti-Human 4-1BB Receptor containing 2.5mg D-mannitol.
Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml.

Human 4-1BB Receptor Standard:
1ug of recombinant Human 4-1BB Receptor containing 2.2mg BSA, 11.0mg D-mannitol.
Centrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 1ug/ml.

Note:
The reconstituted components are stable for 2 weeks when stored at 2-8°C.
Components that have been reconstituted and aliquoted can be stored at -20°C for up to 6 months.

Avidin-HRP Conjugate:
60ul vial. Upon receipt, Avidin-HRP conjugate should be aliquoted into ten 6ul vials and
stored at -20°C or lower. Aliquots stored frozen at below -20°C are stable for up to 2 years from date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only.

Buffer Kit Components:
ELISA microplates, 10x96 well
Sealing Film
PBS
Wash Buffer
Blocking Buffer
Diluent
ABTS Liquid Substrate

Expiration Date:
One year from date of receipt

Note:
Additional buffers can be purchased with our ELISA Buffer Kit: Cat.# 145859
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.