Technical Data
149671
MCP-1, Recombinant, Mouse (C-C Motif Chemokine 2, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic Protein 1, MCP-1, Platelet-derived Growth Factor-inducible Protein JE, Small-inducible Cytokine A2, Ccl2, Je, Mcp1, Scya2)
2ug
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross reacts across species. Recombinant murine JE is a 13.8kD protein containing 125aa including the four highly conserved cysteine residues present in the CC chemokines.

Source:
Recombinant protein corresponding to mouse MCP-1, expressed in E.coli.

Biological Activity:
Determined by its ability to chemoattract Balb/C mouse spleen MNCs using a concentration range of 1-20ng/ml.

Endotoxin:
<0.1ng/ug (1EU/ug)

AA Sequence:
QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: ~98% (SDS-PAGE and HPLC)
Concentration: As reported
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.