Technical Data
A0000-10
A20 Zinc Finger Protein
Description:
NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-kB gene expression. A20 inhibits NF-kB dependent gene expression by interfering with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IkB kinase pathway. A20 is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Intracellular
Western Blot: 2-4ug/ml
Immunoprecipitation: 1-2ug/ml
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cells, THP1

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k4H16Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant fusion protein of full length human A20 Zinc Finger Protein. Epitope: C-terminal portion of A20 (aa440-790).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human A20 Zinc Finger Protein. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological Application References: 1. Wang, J. et al., J. Immunology 183: 1384–1392 (2009) General References: 1. May, M.J. and Ghosh, S., Seminars in Cancer Biology 8(2): 63-73 (1997). 2. Opipari, A.W., et al., J. Biol. Chem. 265: 14,705-14,708 (1990). 3. Heyninck, K., et al., J. Cell Biol. 145(7): 1471-1482 (1999). 4. Tewari, M., et al., J. Immunol. 154: 1699-1706 (1995). 5. Kupfner, J.G., et al., J. Immunol. 167: 7044-7051 (2001). 6. Bohgaki, M., et al., Biochimica et Biophysica Acta 1783: 826-837 (2008). 7. Hailfinger, S., et al., PNAS USA 106: 19,946-19,951 (2009). 8. Coomaert, B., et al., Nature Immunol. 9: 263-271 (2008).