Technical Data
A20 Zinc Finger Protein
NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-kB gene expression. A20 inhibits NF-kB dependent gene expression by interfering with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IkB kinase pathway. A20 is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues.

Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Intracellular
Western Blot: 2-4ug/ml
Immunoprecipitation: 1-2ug/ml
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat cells, THP1

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k4H16Affinity Purified
100ug-20CBlue IceHumanMouse
Recombinant fusion protein of full length human A20 Zinc Finger Protein. Epitope: C-terminal portion of A20 (aa440-790).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.05% sodium azide.
Recognizes human A20 Zinc Finger Protein. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological Application References: 1. Wang, J. et al., J. Immunology 183: 13841392 (2009) General References: 1. May, M.J. and Ghosh, S., Seminars in Cancer Biology 8(2): 63-73 (1997). 2. Opipari, A.W., et al., J. Biol. Chem. 265: 14,705-14,708 (1990). 3. Heyninck, K., et al., J. Cell Biol. 145(7): 1471-1482 (1999). 4. Tewari, M., et al., J. Immunol. 154: 1699-1706 (1995). 5. Kupfner, J.G., et al., J. Immunol. 167: 7044-7051 (2001). 6. Bohgaki, M., et al., Biochimica et Biophysica Acta 1783: 826-837 (2008). 7. Hailfinger, S., et al., PNAS USA 106: 19,946-19,951 (2009). 8. Coomaert, B., et al., Nature Immunol. 9: 263-271 (2008).