Technical Data
A33 (GPA33)
Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain.

Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 50ug/ml. 10ul labels 1-2.5x10e5 HT-29 cells in a reaction volume not exceeding 200ul.
Direct ELISA: 0.5-1.0ug/ml; the detection limit is ~0.2ng/well.
Western Blot: 0.1-0.2ug/ml; The detection limit is ~2ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 2-15ug/ml detects A33 in cells and tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
~0.2mg/ml (after reconstitution)
Purified, recombinant protein corresponding aa22-235, extracellular domain, of human A33 expressed in NSO cells (Q99795). Species Sequence Homology: canine; 80%, bovine; 74%, mouse; 71%.
Purified by affinity chromatography
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Recognizes human A33. A33 is likely to be involved in cell-cell adhesion between epithelial cells. Crossreacts with recombinant mouse A33 ~10%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.