Technical Data
A0000-33B
A33 (GPA33, Glycoprotein A33 (transmembrane) Cell surface A33 antigen precursor, Glycoprotein A33, MGC129986, MGC129987)
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Technical Data |
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A0000-33B |
A33 (GPA33, Glycoprotein A33 (transmembrane) Cell surface A33 antigen precursor, Glycoprotein A33, MGC129986, MGC129987) |
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Description: Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214aa in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug stains 10e6 cells from the HT-29 human colon carcinoma cell line. Western Blot: 1ug/ml Immunohistochemistry: 8-25ug/ml stains immersion fixed paraffin-embedded sectionf of human colon and human colon cancer tissue subjected to antigen retrieval, basic. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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