Technical Data
A0000-33C
A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor, MGC129986, MGC129987) (Biotin)
Description:
Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml
Flow Cytometry: 50ug/ml. Use 10ul of diluted solution/1-2.510e5 cells in <200ul total volume.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.05mg/ml
Immunogen:
Recombinant corresponding to aa22-235 of human A33 extracellular domain expressed inNSO cells (Q99795).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS with BSA. Labeled with Biotin. Reconstitute with 1ml TBS, pH 7.3, 0.1% BSA.
Specificity:
Recognizes human A33. Species crossreactivity: ~10% crossreactivity with recombinant mouse A33.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.