Technical Data
A4GNT (Alpha-1,4-N-acetylglucosaminyltransferase, Alpha4GnT, MGC149493)
This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.

Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:8000
Western Blot: 1-3ug/ml ~40kD band observed in lysates of cell line K562 (calculated MW of 39.5kD according to NP_057245.1)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-PRTYRDLIKGPEGS, from the C-Terminus of human A4GNT (NP_057245.1).
Purified by peptide affinity chromatography.
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Recognizes human A4GNT. Species sequence homology: canine, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ishizone S, Yamauchi K, Kawa S, Suzuki T, Shimizu F, Harada O, Sugiyama A, Miyagawa S, Fukuda M, Nakayama J, Clinical utility of quantitative RT-PCR targeted to alpha1,4-N-acetylglucosaminyltransferase mRNA for detection of pancreatic cancer. Cancer science 2006 Feb 97 (2): 119-26.