		Technical Data
A0001-35	A4GNT (Alpha-1,4-N-acetylglucosaminyltransferase, Alpha4GnT, MGC149493)

Description:
This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:8000
Western Blot: 1-3ug/ml ~40kD band observed in lysates of cell line K562 (calculated MW of 39.5kD according to NP_057245.1)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-PRTYRDLIKGPEGS, from the C-Terminus of human A4GNT (NP_057245.1).

Purity:

Purified by peptide affinity chromatography.

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Specificity:

Recognizes human A4GNT. Species sequence homology: canine, bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ishizone S, Yamauchi K, Kawa S, Suzuki T, Shimizu F, Harada O, Sugiyama A, Miyagawa S, Fukuda M, Nakayama J, Clinical utility of quantitative RT-PCR targeted to alpha1,4-N-acetylglucosaminyltransferase mRNA for detection of pancreatic cancer. Cancer science 2006 Feb 97 (2): 119-26.