Technical Data
A431+ Calyculin A (30min) Lysate
Biologicals Storage: -20CShipping: Blue Ice
Calyculin A is a serine/threonine phosphatase inhibitor that inhibits the activity of protein phosphatases PP1 and PP2A. Human carcinoma A431 cells treated with calyculin A for 30 minutes can undergo significant threonine phosphorylation, as shown by western blotting using anti-Phospho-Akt (Thr-34), as compared to untreated, control cell lysates.
Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated or treated with Calyculin A at a final concentration of 10nM for 30 minutes at 37C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

SDS-PAGE/Western blotting 20ml/well
End user should determine optimal quantity for their particular applications and experiments.
Concentration: ~1mg/ml
Form: Supplied electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1999 Resjo, S. et al. Biochem. J. 341:839-845.
1989 Ishihara, H. et al. Biochem. Biosphys. Res. Commun. 159(3):871-877.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.