Technical Data
A431+ EGF (5min) Lysate
Biologicals Storage: -20CShipping: Blue Ice
A431 cells express approximately 10e6 epidermal growth factor (EGF) receptors at the cell surface. Upon stimulation with EGF, A431 cells exhibit a dramatic increase in phosphorylation of EGF receptors followed by activation of major cell signaling pathways, such as PKB/Akt and MAP kinase pathways. Downstream of these cell signaling pathways, a variety of cytoskeletal, cytoplasmic, and nuclear proteins become phosphorylated at different time points after EGF stimulation.

Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated or treated with human EGF (100 ng/ml) for 30 minutes at 37C.

Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Suitable for use in SDS-PAGE/Western Blot .

Recommended Dilution:
Western Blot: 20ul/well
Optimal dilutions to be determined by the researcher
Storage and Stability:

For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration: ~1mg/ml
Form: Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1999 Resjo, S. et al. Biochem. J. 341:839-845.
1989 Ishihara, H. et al. Biochem. Biophys Res Comm 159(3):871-877.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.