		Technical Data
A0001-95H	AAK1 (AP2-associated Protein Kinase 1, Adaptor-associated Kinase 1, KIAA1048)

Description:
AAK1 is a serine/threonine kinase that is thought to coordinate the recruitment of AP-2 to receptors containing tyrosine-based internalization motifs by phosphorylating the mu2 subunit. There is strong evidence that this protein is the endogenous mu 2 kinase and plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa850 from human AAK1.

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

Specificity:

Recognizes human AAK1 at ~95kD. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.