Technical Data
A0004-01A
ABCA1 (ABC 1, ABC Transporter 1, ATP-binding Cassette 1, ATP-binding Cassette Transporter 1, CERP, Cholesterol Efflux Regulatory Protein, HDLDT1, TGD)
Description:
The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABCA subfamily, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease. Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:200; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a10B15Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
ABCA1 transfected HeLa cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function. Pflugers Arch.453:569-579.