Technical Data
A0004-01A5
ABCA1 (ATP-binding Cassette Sub-family A Member 1, ATP-binding Cassette Transporter 1, ABC-1, ATP-binding Cassette 1, Cholesterol Efflux Regulatory Protein, ABC1, CERP)
Description:
ABCA1 is a 240-280kD member of the ABCA family, ABC transporter superfamily of molecules. It is widely expressed, particularly by macrophages, where it participates in the transport of lipid across cell membranes. Once transported, it is passed on to tethered apoA-I to form HDL. Human ABCA1 is 2261aa in length. It has up to 15 transmembrane segments with beginning and ending cytoplasmic domains and a large cytoplasmic central region. ABC transporters are found between aa899-1311 and aa1912-2144, aa2216-2221 are essential for lipid transport, and two intramolecular disulfide bonds are required for activity aa75-309 and aa1463/1465-1477). There are three potential isoform variants. One possesses an alternative start site at 61aa, while two others contain 12aa and 123aa substitutions for aa241-2261, respectively. Over aa1248-1350, human ABCA1 shares 97% aa identity with mouse ABCA1.

Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2.5ug labels 10e6cells
Immunocytochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
~0.1mg/ml after reconstitution
Immunogen:
Recombinant corresponding to aa1248-1350 from human ABCA1 expressed in E.coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity:
Recognizes human ABCA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.