Technical Data
A0004-01C-P
ABCB5 (ATP-binding cassette, sub-family B MDR/TAP, ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5) (Control Peptide)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
C-QTQHRNTSKKAQ, from the internal region of the protein sequence according to NP_848654.3.
Control peptide corresponding to polyclonal antibody, Catalog#A0004-01C, goat x human.

ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90kD (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812aa in length. It is possibly a five transmembrane protein with a 247aa N-terminal extracellular domain (ECD) aa1-247 and a 283aa C-terminal cytoplasmic region aa530-812. The ECD contains one ABC transporter aa2-177, while the cytoplasmic region contains a second ABC transporter aa570-808. There are two potential splice forms. One shows a seven aa substitution for aa125-812, while another possesses an alternative start site 445aa upstream of the standard site. Over aa141-247, human ABCB5 shares 64aa identity with mouse ABCB5.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking (see corresponding antibody A0004-01C):
Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Purity: Highly Purified
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder in PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma., Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH., Cancer Res. 2005 May 15;65(10):4320-33.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.