Technical Data
A0004-01L
ABCB5 (ATP-binding Cassette Sub-family B Member 5, ABCB5 P-gp, P-glycoprotein ABCB5)
Description:
ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90kD (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812aa in length. It is possibly a five transmembrane protein with a 247aa N-terminal extracellular domain (ECD) aa1-247 and a 283aa C-terminal cytoplasmic region aa530-812. The ECD contains one ABC transporter aa2-177, while the cytoplasmic region contains a second ABC transporter aa570-808. There are two potential splice forms. One shows a seven aa substitution for aa125-812, while another possesses an alternative start site 445aa upstream of the standard site. Over aa141-247, human ABCB5 shares 64aa identity with mouse ABCB5.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa141-247 from human ABCB5 expressed in E. coli (Q2M3G0).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity:
Recognizes human HABCB5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.